Thermo Fisher Scientific ABH1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 110-389 of human ALKBH1 (NP_0060112) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ABH1,uniProtId:Q13686-1,ncbiNodeId:9606,antigenRange:110-389,antigenLength:389,antigenImageFileName:PA5-89613_ABH1_Q13686-1_Rabbit.svg,antigenImageFileNamePDP:PA5-89613_ABH1_Q13686-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.01 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805688

Product Specific Information

Immunogen sequence: YQWHWVKQCL KLYSQKPNVC NLDKHMSKEE TQDLWEQSKE FLRYKEATKR RPRSLLEKLR WVTVGYHYNW DSKKYSADHY TPFPSDLGFL SEQVAAACGF EDFRAEAGIL NYYRLDSTLG IHVDRSELDH SKPLLSFSFG QSAIFLLGGL QRDEAPTAMF MHSGDIMIMS GFSRLLNHAV PRVLPNPEGE GLPHCLEAPL PAVLPRDSMV EPCSMEDWQV CASYLKTARV NMTVRQVLAT DQNFPLEPIE DEKRDISTEG FCHLDDQNSE VKRARINPDS; Positive Samples: 293T; Cellular Location: Mitochondrion, Nucleus

Target Information

This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation. Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.