Thermo Fisher Scientific HSF1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody is predicted to react with bovine based on sequence homology.

Target Information

All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are composed of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation and are present upstream of all heat shock genes.
Although HSEs are highly conserved, HSF proteins purified from yeast, Drosophila, and human differ in molecular weight and show limited immunological cross-reactivity. Two HSFs have been identified in human cells: HSF1 and HSF2, which bind the same HSEs and share 38% sequence identity.
HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA-binding activity. Upon stress, HSF1 becomes activated into a nuclear-localized trimeric form that binds DNA. Phosphorylation of HSF1 is required for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.