Thermo Fisher Scientific CD19 Monoclonal Antibody (SJ25C1), NovaFluor Yellow 590, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (SJ25C1), NovaFluor Yellow 590, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Product Specific Information

The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed on B cells during all stages of development except terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together with CD21, CD81, Leu13, and MHC class II, CD19 forms a multimolecular complex associated with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B cell proliferation. SJ25C1 and HIB19 antibodies recognize overlapping epitopes.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.8 µg)/test. A test is defined as staining a cell sample in 100 µL final volume. Recommended titration for optimal assay performance.

NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.

This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which improves formulation for optimal staining. Add 5 µL per sample to antibody cocktails before combining with cells. For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures, engineered for optimized fluorescent signatures with minimized spillover and spread.

Excitation: 552 nm
Emission: 592 nm
Laser: 561 nm (Yellow)

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and may appear on certain myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor for the BCR, regulating normal and malignant B cell proliferation. Mutation causes hypogammaglobulinemia; overexpression leads to B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Available Conjugate Formats

• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805 series
• Brilliant Violet 605, 650
• eFluor 450, 506
• NovaFluor Blue, Red, Yellow series
• PE, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780 series
• Custom conjugation available upon request

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