Thermo Fisher Scientific beta Catenin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:250

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

5 µg/ml

-

Product Specifications

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from the C-terminus of the beta-catenin protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:beta Catenin,uniProtId:P35222-1,ncbiNodeId:9606,antigenRange:781,antigenLength:781,antigenImageFileName:PA5-16762_beta_Catenin_P35222-1_Rabbit.svg,antigenImageFileNamePDP:PA5-16762_beta_Catenin_P35222-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.07 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10980908

Product Specific Information

This antibody reacts with the C-terminal portion of beta-Catenin.

For staining of formalin-fixed, paraffin-embedded tissues, it is recommended to boil tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at room temperature for 20 min. Recommended positive controls are MCF-7 cells and breast carcinoma tissue.

This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Target Information

Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.