Thermo Fisher Scientific beta Catenin Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody reacts with the C-terminal portion of beta-Catenin.
For staining of formalin-fixed, paraffin-embedded tissues, it is recommended to boil tissue sections in 10 mM citrate buffer (pH 6.0) for 10–20 minutes, followed by cooling at room temperature for 20 minutes.
Recommended positive controls are MCF-7 cells and breast carcinoma tissue.

This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Target Information

Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells.

Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton.
It also binds to complexes containing adenomatous polyposis coli protein and microtubules, and participates in signaling pathways involving tyrosine kinases, phosphatases, and Wnt/Wingless.

Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheaths.
Pathological roles have been identified in pilomatrixoma, medulloblastoma, colorectal cancer, ovarian cancer, and other tumor types.

In the nucleus, beta-catenin co-activates Lef/Tcf transcription factors, stimulating transcription of target genes such as cyclin D and c-myc, which promote cell proliferation and tumorigenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.