Thermo Fisher Scientific CAST Monoclonal Antibody (OTI1E7), TrueMAB
Calpastatin 단백질을 인식하는 마우스 단클론 항체로 Western blot, IHC, Flow cytometry에 사용 가능. 인간 시료 반응성, 고순도 친화 크로마토그래피 정제, 동결건조 형태로 안정적 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E7 |
| Immunogen | Full length human recombinant protein of human CAST produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Calpastatin is an endogenous inhibitor of calpain, a calcium-dependent cysteine protease. It contains one N-terminal domain and four repetitive calpain-inhibition domains (1–4). This protein participates in amyloid precursor protein proteolysis and various membrane fusion events such as neural vesicle exocytosis and platelet aggregation. Calpastatin may also influence the expression of genes encoding structural or regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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