Thermo Fisher Scientific TCL1 Recombinant Rabbit Monoclonal Antibody (TCL1, 2747R)
Recombinant Rabbit Monoclonal Antibody targeting TCL1 protein. Validated for IHC (Paraffin) with high specificity to human TCL1. Produced in HEK293 cells and purified via Protein A/G. Supplied as a liquid, 200 µg/mL in PBS with 0.05% BSA and sodi...
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Applications
Tested Application and Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | TCL1, 2747R |
| Immunogen | Recombinant human TCL1 protein fragment (around aa 2–109) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Overexpression of the TCL1 gene in humans has been implicated in the development of mature T-cell leukemia, where chromosomal rearrangements bring the TCL1 gene close to T-cell antigen receptor (TCR)-alpha or TCR-beta regulatory elements (Virgilio et al., 1998).
In normal T cells, TCL1 is expressed in CD4-/CD8- cells but not in cells at later differentiation stages.
TCL1 functions as a coactivator of the cell survival kinase AKT (Laine et al., 2000).
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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