Thermo Fisher Scientific CD14 Monoclonal Antibody (MEM-15), NovaFluor Yellow 610, eBioscience

Thermo Fisher Scientific CD14 Monoclonal Antibody (MEM-15), NovaFluor Yellow 610, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test
Publications: -

Product Specifications

Product Specific Information

The MEM-15 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages, and some dendritic cells. Complexes of LPS and LBP bind with high affinity to monocytes through surface CD14.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated by flow cytometric analysis of normal human peripheral blood cells.
Recommended usage: 5 µL (0.6 µg) per test in a final volume of 100 µL (10⁵–10⁸ cells/test).

NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI.
Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended.

This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which provides improved formulation for optimal staining.
Use 5 µL per sample in antibody cocktails or single-color controls.

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimal fluorescence with minimized spectral spillover.
Excitation: 552 nm; Emission: 612 nm; Laser: 561 nm (Yellow).

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein constitutively expressed on mature monocytes, macrophages, and neutrophils. It acts as a multifunctional lipopolysaccharide receptor and exists in both membrane-bound and soluble forms. CD14 binds LPS in a reaction catalyzed by LBP and plays a role in immune response modulation, apoptosis recognition, and inflammatory processes.
Diseases associated with CD14 dysfunction include Mycobacterium chelonae infection and Croup.

For Research Use Only. Not for use in diagnostic procedures.

Related NovaFluor Formats

• NovaFluor Blue 510
• NovaFluor Blue 530
• NovaFluor Blue 585
• NovaFluor Blue 610-30S
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 660-40S
• NovaFluor Red 700
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 690
• NovaFluor Yellow 700
• NovaFluor Yellow 730
• Request custom conjugation

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