Thermo Fisher Scientific Phospho-BLNK (Tyr84) Polyclonal Antibody
Phospho-BLNK (Tyr84) 폴리클로날 항체로, 인간 및 생쥐 시료에서 BLNK 단백질의 인산화 상태를 검출. Western blot 및 IHC(파라핀)에서 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체로, 안정적인 액상 형태로 제공. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to a portion of human BLNK containing tyrosine 84. The sequence is conserved in mouse. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_779406 |
Product Specific Information
- Recommended positive controls: EBI cells ± H₂O₂ treatment
- PA1-26639 is expected to cross-react with chicken (83% conserved) and mouse (100% conserved) due to sequence homology.
- The immunogen is a synthetic phosphopeptide corresponding to human BLNK containing tyrosine 84, conserved in mouse.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
B cell linker protein (BLNK) is encoded by the BLNK gene located on chromosome 10 in humans. It is also known as SLP-65 (Src homology 2 domain-containing leukocyte protein of 65 kDa). BLNK functions as an adaptor protein in the B cell receptor signaling cascade. In the absence of BLNK, B cell development is impaired. BLNK undergoes tyrosine phosphorylation by Syk, a B cell receptor-activated kinase, enabling interaction with signaling intermediates such as Vav GEF and Grb2.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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