Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (TS2/16), Super Bright 645, eBioscience

Applications

• Tested Application: Flow Cytometry (Flow)
• Recommended Dilution: 5 µL (0.125 µg)/test
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Product Specifications

Available Formats

• Unconjugated
• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

Description:
The TS2/16 monoclonal antibody reacts with human CD29 (Integrin beta 1), a ~130 kDa transmembrane glycoprotein forming heterodimers with integrin alpha subunits to create the VLA subfamily of adhesion molecules. CD29 participates in cell-cell and cell-matrix adhesion and is expressed on lymphocytes and monocytes, with lower levels on granulocytes. The TS2/16 clone exhibits activating activity for beta 1 integrins.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested by flow cytometry on normal human peripheral blood cells. Recommended use: 5 µL (0.125 µg) per test in 100 µL final volume, with 10⁵–10⁸ cells/test.

Fluorochrome Information:
Super Bright 645 is a tandem dye excited by the violet laser (405 nm) and emits at 645 nm. Use a 660/20 bandpass filter. Ensure compatibility with instrument configuration.

Staining Recommendations:
For panels using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-Step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation / Emission / Laser:
405 nm / 645 nm / Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Gene Symbol: ITGB1 (Integrin Subunit Beta 1, CD29)
Protein Size: 110 kDa
Expression: Broadly expressed on leukocytes, platelets, and various tissues.
Function: Forms heterodimers with multiple alpha chains (α1–α6) to mediate cell adhesion and signaling. Plays roles in immune cell trafficking, tissue remodeling, and inflammatory response regulation.
Clinical Relevance: Altered expression is linked to multidrug resistance in tumors and inflammatory conditions such as IBD. Two major isoforms, β1A and β1D, show tissue-specific expression patterns.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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