Thermo Fisher Scientific Phospho-Smad1 (Ser453, Ser455) Polyclonal Antibody, FITC
Phospho-Smad1 (Ser453, Ser455) 인산화 단백질을 인식하는 FITC 결합 토끼 다클론 항체입니다. Western blot, ELISA, IP에 사용 가능하며, Drosophila 단백질에 반응합니다. Affinity chromatography로 정제되었으며, -20°C 어둠 속 보관이 권장됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fruit fly |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 405–455 of D. melanogaster Mothers against dpp protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The SMAD1 protein belongs to the SMAD family, which shares homology with the Drosophila gene mothers against decapentaplegic (Mad) and C. elegans gene Sma. SMAD proteins act as signal transducers and transcriptional modulators mediating multiple signaling pathways. SMAD1 mediates bone morphogenetic protein (BMP) signaling, influencing processes such as cell growth, apoptosis, morphogenesis, development, and immune responses. Upon BMP ligand binding, SMAD1 is phosphorylated and forms a complex with SMAD4 to regulate transcription. It is also targeted for ubiquitination and degradation by E3 ligases such as SMURF1 and SMURF2. Alternative splicing variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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