Thermo Fisher Scientific Human ALK, GST Tag Recombinant Protein
인간 ALK 단백질을 GST 태그로 발현한 활성 재조합 단백질로, 키나아제 활성 분석에 적합합니다. 곤충 세포에서 발현되었으며 고순도로 정제되었습니다. 뇌 발달 관련 연구 및 종양 관련 ALK 변이 연구용으로 사용됩니다.
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Thermo Fisher Scientific Human ALK, GST Tag Recombinant Protein
Applications
- Kinase Assay (KA)
Tested Dilution: Assay-dependent
Publications: [References not provided]
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | Insect cells |
| Amino Acid Sequence | 1058–1620 |
| Tag | GST-tag |
| Molecular Weight | 89.9 kDa |
| Class | Recombinant |
| Type | Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Purified |
| Storage Buffer | 50 mM Tris HCl, pH 7.5, with 2 mM DTT, 0.02% Triton X-100, 50% glycerol, 150 mM NaCl, 0.5 mM EDTA |
| Contains | No preservative |
| Storage Conditions | -80°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
| Protein Form | Active |
Product Specific Information
- For maximum recovery, spin prior to use.
- Aliquots of 5 µg, 10 µg, and 20 µg sizes are recommended to be used directly from original packaging.
- For larger sizes, aliquoting can reduce freeze/thaw cycles; aliquots below 20 µL are not recommended.
- Do not store kinase diluted.
- Proper storage at -80°C guarantees stability for 6 months from purchase date.
Related Links:
Target Information
Anaplastic Lymphoma Receptor Tyrosine Kinase (ALK) belongs to the insulin receptor superfamily and plays a crucial role in brain development. Mutations, rearrangements, and amplifications of the ALK gene are implicated in various tumors, including anaplastic large cell lymphoma, neuroblastoma, and non-small cell lung cancer.
Chromosomal rearrangement often produces a fusion gene combining ALK and nucleophosmin (NPM), resulting in an oncogenic NPM-ALK product. The ALK protein contains transmembrane and extracellular domains absent in the NPM-ALK fusion. ALK shares high sequence similarity with LTK and influences specific neuronal development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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