Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), Super Bright 600, eBioscience
인간 CD86(B7-2)에 특이적인 IT2.2 클론 단일클론 항체로, Super Bright 600 형광체로 결합되어 Flow Cytometry에 최적화됨. B세포 및 수지상세포의 활성화 연구에 적합하며, 높은 형광 안정성과 낮은 비특이적 결합을 제공. 4°C 암소 보관 권장.
- 카탈로그번호
- 63-0869-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), Super Bright 600, eBioscience
Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), Super Bright 600, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 14 publications |
| Neutralization (Neu) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IT2.2 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2717048 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 647
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 737
- Brilliant Violet 480
- Brilliant Violet 711
- Brilliant Violet 786
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 700
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 610
- NovaFluor Yellow 690
- NovaFluor Yellow 700
- NovaFluor Yellow 755
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 645
- Request custom conjugation
Product Specific Information
Description:
The IT2.2 monoclonal antibody reacts with human CD86 (~80 kDa), also known as B7-2. CD86 and CD80 are costimulatory molecules expressed on B cells, macrophages, and dendritic cells. CD86 interacts with CD28 and CTLA-4 on T cells, playing a key role in T-B cell crosstalk, T cell costimulation, and antibody production.
Applications Reported:
This antibody is reported for use in flow cytometric analysis.
Applications Tested:
Pre-diluted and validated for flow cytometry of normal human peripheral blood cells at 5 µL (0.5 µg) per test. A test is defined as staining a sample in 100 µL volume. Cell numbers may range from 10⁵ to 10⁸ cells/test.
Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Recommended filter: 610/20 bandpass.
Usage Recommendations:
- Use Super Bright Complete Staining Buffer (Product # SB-4401) when combining multiple Super Bright dyes to reduce non-specific interactions.
- Protect from light due to photo-induced oxidation sensitivity.
- Fixed samples can be stored up to 3 days at 4°C using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).
Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD86 is a ligand for CTLA4 and CD28, acting as a costimulatory molecule during antigen presentation. Insufficient co-stimulation can induce tolerance. CD86 is expressed by antigen-presenting cells and is involved in T-cell activation and immune regulation. Alternative splicing produces multiple isoforms. CD86 dysfunction is associated with gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.