Invitrogen™

RNAqueous™-Micro Total RNA Isolation Kit

The RNAqueous™-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from less than 500,000 cultured cells,자세히 알아보기

The RNAqueous™-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from less than 500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-_free_™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. This kit provides:

• Highly concentrated RNA—for increased sensitivity in downstream assays
• Isolation of high-quality RNA from micro-scale samples
• Reagents/protocol for RNA recovery from LCM samples

How it works
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 μL. Residual genomic DNA is efficiently removed using the DNA-_free_™ system included in the kit. The RNAqueous™-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.

사양

Isolation TechnologySilica Spin Column

정제 시간15 min. (After sample homogenization)

용리량10–20 μL

샘플 종류Cells, Tissue, Laser Capture Microdissection (LCM)

최종 제품 유형Total RNA

고처리량 호환성Not High-throughput Compatible (Manual)

용도(애플리케이션)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling

수량50 Preps

배송 조건Box 1: Dry Ice
Box 2: Room Temperature

시작 물질 양Cells: ≤105
Tissue: ≤10 mg

Unit SizeEach