Thermo Fisher Scientific Choline Oxidase Polyclonal Antibody, Biotin
Choline Oxidase를 인식하는 Biotin 결합 폴리클로날 항체로, Western blot, ELISA, IP에 적합합니다. Goat 유래 IgG이며, Alcaligenes species의 Choline Oxidase로 면역화되었습니다. 고순도 정제 및 안정한 보관 조건을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:1,000–1:2,500 |
| Immunoprecipitation (IP) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Choline Oxidase [Alcaligenes species] |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Product is stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Anti-Choline Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis against the specified buffer.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, and purified or partially purified Choline Oxidase [Alcaligenes species].
Cross-reactivity against Choline Oxidase from other sources is unknown.
Target Information
Choline Oxidase catalyzes the reaction:
Choline + 2 O₂ + H₂O → Betaine + 2 H₂O₂
This enzyme is involved in the first step of the subpathway that synthesizes betaine from choline.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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