Thermo Fisher Scientific Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor 488-5 nm colloidal gold

Applications

Tested Dilution

Immunomicroscopy (IM)

0.2-10 µg/mL

Product Specifications

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobin

Conjugate

Alexa Fluor™ 488-5 nm colloidal gold Alexa Fluor™ 488-5 nm colloidal gold Alexa Fluor™ 488-5 nm colloidal gold

Form

Liquid

Concentration

2 mg/mL

Storage conditions

4° C, store in dark

RRID

AB_2536174

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

This 5 nm colloidal gold conjugate may be used as a probe in immunoblotting, light microscopy, fluorescence microscopy or electron microscopy. The fluorescence of this conjugate can be easily detected by standard techniques, but the ease of visualization of colloidal gold can be greatly improved using silver-enhancement methods, such as those we provide in the LI Silver Enhancement Kit (Cat. no. L-24919).

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.