Thermo Fisher Scientific Cul2 Polyclonal Antibody
Cul2 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, IHC, IP 등 다양한 응용에 적합하며 인간 시료에 반응. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 150 and 200 of human cullin 2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Mouse and Orangutan.
Target Information
Cullin 2 (CUL2) is a core component of multiple cullin-RING-based ECS (ElonginB/C–CUL2/5–SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate ubiquitination of target proteins. ECS complexes and ARIH1 collaborate to mediate ubiquitination of target proteins. CUL2 serves as a rigid scaffold in the complex, contributing to catalysis through positioning of the substrate and ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity is dependent on neddylation of the cullin subunit and inhibited by deneddylated cullin associated with TIP120A/CAND1. ECS(VHL) mediates ubiquitination of hypoxia-inducible factor (HIF).
[Source: UniProt]
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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