Thermo Fisher Scientific hnRNP C1/C2 Monoclonal Antibody (4F4)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

RNA Immunoprecipitation (RIP)

1.25 µg/10^6 cells

-

Product Specifications

Species Reactivity

Chicken, Hamster, Human, Non-human primate

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4F4

Immunogen

RNP`s eluted from oligo (dT) cellulose column.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.4 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_794497

Product Specific Information

Recommended positive controls: HeLa nuclear extract.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. hnRNP C1 and C2 are encoded by one gene; the two alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.