Thermo Fisher Scientific SSEA1 Monoclonal Antibody (MC-480)
SSEA1 (CD15) 항원을 인식하는 Mouse IgM 단일클론 항체로, 인간 및 마우스 시료에 반응합니다. IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합하며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 | View 3 publications |
| Flow Cytometry (Flow) | 1:100 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse, Pig |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MC-480 |
| Immunogen | BALB/c mouse immunized with F9 teratocarcinoma stem cells (X-irradiated) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography - MBP |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536693 |
Available Conjugated Formats
- DyLight 488 (MA1-022-D488)
- DyLight 550 (MA1-022-D550)
- DyLight 650 (MA1-022-D650)
- PE (MA1-022-PE)
- Request custom conjugation
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on various glycolipids, glycoproteins, and proteoglycans across cell types such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X.
Cells with high Le(x) expression exhibit calcium-dependent self-aggregation, involved in embryo compaction and teratocarcinoma cell autoaggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands.
CD15 expression is widely used to confirm Hodgkin’s disease diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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