Thermo Fisher Scientific VMA2 Monoclonal Antibody (13D11B2)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This clone recognizes the 60 kDa subunit 1 (B-subunit, the product of the VMA2 or VAT2 gene) of S. cerevisiae.
By Western blot, it can be used to identify organelles during subcellular fractionation of yeast membranes.

Target Information

Vacuolar-type H⁺-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles.
V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process to synthesize ATP.
The enzyme consists of:

• Peripheral V1 domain (ATP hydrolysis)
• Integral V0 domain (proton translocation)

The V1 domain has nine subunits (A–H), and the V0 domain has five subunits (a, d, c, c′, c″).
Like F-ATPase, V-ATPase likely operates through a rotary mechanism.

The V-ATPase V1 B subunit exists as two isoforms:

• B1 isoform: functions in proton secretion in the inner ear, maintaining endolymph pH and auditory function.
  Mutations in this gene cause distal renal tubular acidosis with sensorineural deafness.
• B2 isoform: expressed in kidney and osteoclasts (only B isoform in osteoclasts).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.