Thermo Fisher Scientific VMA2 Monoclonal Antibody (13D11B2)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25 µg/mL

View 23 publications 23 publications

Immunocytochemistry (ICC/IF)

20 µg/mL

View 9 publications 9 publications

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 14 publications 14 publications

Product Specifications

Species Reactivity

Yeast

Published species

Yeast

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

13D11B2

Immunogen

Purified S. cerevisiae vacuole membranes

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2536202

Product Specific Information

This clone recognizes the 60 kDa subunit 1 (B-subunit, the product of the VMA2 or VAT2 gene) of S. cerevisiae. By Western blot, this can be used to identify organelles during subcellular fractionation of yeast membranes.

Target Information

Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, cand c) make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. The V-ATPase V1 B subunit exists as two isoforms. In the inner ear, the V-ATPase B1 isoform functions in proton secretion and is required to maintain proper endolymph pH and normal auditory function. The gene encoding the human V-ATPase B1 isoform maps to chromosome 2cen-q13. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. The V-ATPase B2 isoform is expressed in kidney and is the only B isoform expressed in osteoclasts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.