
Thermo Fisher Scientific PYCRL Monoclonal Antibody (OTI8H6), TrueMAB
인간 PYCRL 단백질을 인식하는 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8H6 |
| Immunogen | Full length human recombinant protein of PYCRL produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
PYCRL encodes a protein belonging to the pyrroline-5-carboxylate reductase family of enzymes.
These enzymes catalyze the final step in proline biosynthesis, converting pyrroline-5-carboxylate to proline.
Glutamate and ornithine serve as precursors for proline synthesis.
The PYCRL protein functions as a cytoplasmic enzyme involved in the biosynthesis of proline from ornithine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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