Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), NovaFluor Red 700, eBioscience

Applications

• Tested Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.05 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 647, 700
• APC, Biotin
• Brilliant UV 737, Brilliant Violet 480, 711, 786
• Functional Grade
• NovaFluor Blue 660-120S, Red 725, Red 755, Yellow 610, Yellow 690, Yellow 700, Yellow 755
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436, 600, 645
• Custom conjugation available

Product Specific Information

Description:
The IT2.2 monoclonal antibody reacts with human CD86 (B7-2), an ~80 kDa surface receptor. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and is upregulated upon stimulation through BCR, CD40, and cytokine receptors.

CD86 interacts with CD28 and CTLA-4 on T cells, playing a key role in T-B cell crosstalk, T cell costimulation, and immune regulation. Its rapid upregulation supports its major role during the primary immune response.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL/test (0.05 µg/test).
A test is defined as the amount of antibody required to stain a 100 µL cell sample (10⁵–10⁸ cells/test).

Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton technology based on nucleic acid dye structures, engineered for optimized fluorescence with minimal spillover and spread.

Excitation: 639 nm
Emission: 700 nm
Laser: 633–640 nm (Red)

Target Information

CD86 (B7-2) is a ligand for CTLA4 and CD28, acting as a costimulatory molecule during antigen presentation.

• Function: Provides T-cell activation signals via CD28 binding; inhibits activation via CTLA-4 binding.
• Expression: Antigen-presenting cells (APCs) such as B cells, macrophages, and dendritic cells.
• Clinical Relevance: Dysregulation associated with gallbladder squamous cell carcinoma and myocarditis.
• Structure: Type I membrane protein, member of the immunoglobulin superfamily.
• Variants: Alternative splicing yields multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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