Thermo Fisher Scientific Occludin Polyclonal Antibody
Occludin 단백질을 인식하는 Rabbit Polyclonal Antibody로 다양한 종에서 반응성을 보임. Western blot, IHC, ICC/IF 등 여러 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 PBS buffer에 보관. 연구용 전용 제품.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1.5–2 µg/mL | View 171 publications |
| Immunohistochemistry (IHC) | Assay-dependent | View 80 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 24 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 10 publications |
| Immunohistochemistry - Free Floating (IHC (Free)) | – | View 2 publications |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | View 92 publications |
| ELISA | Assay-dependent | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | View 4 publications |
| Miscellaneous PubMed (Misc) | – | View 28 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Published Species | Avian, Baboon, Bacteria, Bovine, Chicken, Dog, Goat, Hamster, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A fusion protein containing the C-terminal 150 amino acid region of human occludin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533977 |
Target Information
Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. It is involved in regulating both localization and function of tight junctions. Polyunsaturated fatty acids up-regulate occludin expression, increasing transendothelial resistance and reducing permeability to large molecules. Expression levels vary by tissue — highly expressed in brain tissue at cell-cell contact sites and lower in non-neural tissues. Structural features are conserved across species. Under-expression of occludin contributes to increased permeability in tumor endothelial junctions, leading to brain tumor edema.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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