Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (3DS223H), Alexa Fluor 660, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.25 µg)/test

Product Specifications

Available Conjugate Formats

• Alexa Fluor 647
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395 / 563 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450 / 506
• FITC
• NovaFluor Blue 510 / 660-120S
• NovaFluor Red 700
• NovaFluor Yellow 590 / 610 / 700 / 730
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The 3DS223H monoclonal antibody recognizes human CD223, also known as Lymphocyte Activation Gene 3 (LAG-3). LAG-3 is a 70-kDa surface glycoprotein in the Ig superfamily, homologous to CD4. It binds to MHC class II with higher affinity than CD4 and is involved in the negative regulation of T cell activation and homeostatic proliferation.

Surface expression of LAG-3 is found on activated T cells (including regulatory T cells) and NK cells. CD8⁺ T cells typically express higher levels of LAG-3 than CD4⁺ T cells. Coexpression of LAG-3 and CD49b has been proposed as a marker of Type 1 regulatory T cells (Tr1 cells).

• Recognizes a formaldehyde-fixed epitope
• Reported application: Flow cytometric analysis
• Tested on stimulated normal human peripheral blood cells
• Recommended use: 5 µL (0.25 µg) per test in 100 µL final volume
• Alexa Fluor 660 emits at 690 nm and is suitable for spectral cytometers
• Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser

Target Information

LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. It binds to MHC class II with higher affinity than CD4 and functions in negative regulation of T cell activation and proliferation. Expression is observed on activated T cells (including regulatory T cells) and NK cells, with higher expression in CD8⁺ T cells. Coexpression with CD49b identifies Type 1 regulatory T cells (Tr1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지