Thermo Fisher Scientific UBE2O Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human UBE2O. Recombinant protein control fragment (Product #RP-102086). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:UBE2O,uniProtId:Q9C0C9-1,ncbiNodeId:9606,antigenRange:130-254,antigenLength:1292,antigenImageFileName:PA5-54839_UBE2O_Q9C0C9-1_Rabbit.svg,antigenImageFileNamePDP:PA5-54839_UBE2O_Q9C0C9-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.10 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2649183

Product Specific Information

Immunogen sequence: GVKQHVKETK LKLEDRSVVP RDVVRHMRST DSQCGTVIDV NIDCAVKLIG TNCIIYPVNS KDLQHIWPFM YGDYIAYDCW LGKVYDLKNQ IILKLSNGAR CSMNTEDGAK LYDVCPHVSD SGLFF

Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 79%.

Target Information

E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate Lys-63-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.