Thermo Fisher Scientific UBE2O Polyclonal Antibody
Thermo Fisher Scientific의 UBE2O Polyclonal Antibody는 인간 UBE2O 단백질을 인식하는 토끼 폴리클로날 항체입니다. Western blot 및 면역세포화학(ICC/IF)에 적합하며, 고순도의 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human UBE2O. Recombinant protein control fragment (Product #RP-102086) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.10 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2649183 |
Product Specific Information
Immunogen sequence:
GVKQHVKETK LKLEDRSVVP RDVVRHMRST DSQCGTVIDV NIDCAVKLIG TNCIIYPVNS KDLQHIWPFM YGDYIAYDCW LGKVYDLKNQ IILKLSNGAR CSMNTEDGAK LYDVCPHVSD SGLFF
Highest antigen sequence identity to the following orthologs:
- Mouse: 100%
- Rat: 79%
Target Information
E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950).
Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138).
Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153).
Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1.
Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950).
Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate Lys-63-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853).
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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