Thermo Fisher Scientific Cytokeratin 18 (KRT18) Monoclonal Antibody (KRT18/835)
인간 Cytokeratin 18 단백질을 인식하는 마우스 단클론 항체입니다. Western blot, IHC, ICC 등 다양한 응용에 사용 가능합니다. Rat, Sheep, Hamster, Cow, Dog, Pig에는 반응하지 않습니다. 단백질 A/G로 정제된 액상 항체로 4°C 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | KRT18/835 |
| Immunogen | Recombinant full-length human KRT18 protein (Cytokeratin 18, UniProt ID: P05783-1, aa 1–430) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Rat, Sheep, Hamster, Cow, Dog, or Pig.
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with cytokeratin 8. It is expressed in tissues from the gastrointestinal, respiratory, and urogenital tracts, as well as in endocrine, exocrine, and mesothelial cells. Cytokeratin 18 is found mainly in non-squamous epithelia and is present in most adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been identified. Cytokeratin subtype expression patterns are useful for distinguishing different epithelial malignancies. Cytokeratin antibodies assist in tumor differential diagnosis via immunohistochemistry and are valuable tools in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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