Thermo Fisher Scientific POLR3H Polyclonal Antibody
Human POLR3H 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC(P), ICC/IF에 적합. 항원 친화 크로마토그래피로 정제되었으며, 0.3 mg/mL 농도의 액상 형태. RNA polymerase III 관련 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human POLR3H. Recombinant protein control fragment (Product #RP-91548). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2645774 |
Product Specific Information
Immunogen sequence:
GFFDDILIPP ESLQQPAKFD EAEQVWVWEY ETEEGAHDLY MDTGEEIRFR VVDESFVDTS PTGPSSADAT TSSEELPKKE APYTLVG
Highest antigen sequence identity to the following orthologs:
- Mouse: 97%
- Rat: 97%
Target Information
RPC8 is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is a peripheral component of RNA polymerase III, which synthesizes small RNAs such as 5S rRNA and tRNAs.
It plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses, acting as a nuclear and cytosolic DNA sensor involved in the innate immune response. It can sense non-self dsDNA that serves as a template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs), induce type I interferon and NF-κB through the RIG-I pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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