Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBio1D4B (1D4B)), Biotin, eBioscience
마우스 CD107a(LAMP-1)를 인식하는 eBio1D4B 단클론 항체로, Biotin 결합형입니다. Flow cytometry 및 intracellular staining에 적합하며, 0.25 µg/test로 사용 가능합니다. 4°C 암소 보관, Affinity chromatography로 정제된 고품질 항체입니다.
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카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBio1D4B (1D4B)), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 4 publications |
| Immunocytochemistry (ICC/IF) | - | View 11 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 11 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio1D4B (1D4B) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657544 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC-eFluor 780
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The eBio1D4B monoclonal antibody reacts with mouse CD107a (LAMP-1), a type I lysosomal membrane protein that is highly glycosylated. CD107a is constitutively expressed in late endosomes and lysosomes of all cells and transiently expressed on the surface of degranulating cytolytic T cells. It is involved in various cellular functions including cancer metastasis and serves as a marker for lysosomal storage disorders.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis
- Surface staining in flow cytometric degranulation assays
Applications Tested:
- Tested by intracellular staining and flow cytometric analysis of thioglycolate-elicited peritoneal exudate cells
- Recommended usage ≤ 0.25 µg/test (100 µL final volume)
- Cell number range: 10⁵–10⁸ cells/test
- Antibody should be titrated for optimal performance
Target Information
LAMP1 (CD107a), together with LAMP2, is a major lysosomal membrane constituent. Approximately 1–2% of CD107a is also found on the plasma membrane. LAMP1 is a heavily glycosylated type I integral membrane protein containing a signal peptide, 18 N-linked glycosylation sites, a single membrane-spanning segment, and a short cytosolic tail.
LAMP proteins are essential for lysosome biogenesis and fusion with phagosomes. Upon cell activation, LAMP1 translocates to the plasma membrane via a carboxyl-terminal tyrosine-based motif (YXXI). Mutations affecting this motif disrupt lysosome localization.
Cell surface LAMP1 and LAMP2 promote adhesion of PBMCs to vascular endothelium, suggesting involvement in inflammation sites. Increased LAMP1 immunoreactivity is observed in neurons and glial cells near senile plaques in Alzheimer’s disease and in thymic epithelial cells during acute involution. LAMP1 is a reliable marker of mast cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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