
Thermo Fisher Scientific Phospho-SMAD2/SMAD3 (Thr8) Polyclonal Antibody
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Phospho-SMAD2/SMAD3 (Thr8) 폴리클로날 항체로 인간, 마우스, 랫트 시료에 반응. WB, ICC/IF, IP에 사용 가능. 항원 친화 크로마토그래피로 정제되어 고순도(>95%) 확보. TGFβ/BMP 신호전달 연구에 적합.
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Thermo Fisher Scientific Phospho-SMAD2/SMAD3 (Thr8) Polyclonal Antibody
Applications and Tested Dilution
- Western Blot (WB): 1:1,000–1:2,000
- Immunocytochemistry (ICC/IF): 1:50–1:200
- Immunoprecipitation (IP): 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2553332 |
Product Specific Information
- Detects endogenous protein at molecular weights of 55 and 60 kDa.
- Purity: >95% by SDS-PAGE.
Target Information
Smad proteins are mammalian homologs of the Drosophila mothers against decapentaplegic (Mad) and act as downstream effectors of TGFβ/BMP signaling.
- Smad1 and Smad5: Effectors of BMP-2 and BMP-4 functions.
- Smad2 and Smad3: Involved in TGFβ and Activin-mediated growth modulation.
- Smad4: Mediates activities through interaction with various Smad family members.
- Smad6 and Smad7: Regulate Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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