Thermo Fisher Scientific GAPDH Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Keep as concentrated solution.
• Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Cat (100%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%).
• Positive Control: A431, H1299, HeLa, HepG2, Neuro2A, BCL-1, Raw264.7, SH-SY5Y.
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis. It exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH participates in processes such as DNA replication, DNA repair, nuclear RNA export, membrane fusion, and microtubule bundling. It also plays roles in apoptosis and neurodegenerative disease mechanisms.
GAPDH interacts with various proteins, including amyloid precursor protein (linked to Alzheimer’s disease) and Huntingtin (linked to Huntington’s disease). Due to its high expression across tissues, GAPDH is widely used as a protein loading control in Western blot analysis.

Safety Information

⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.