Thermo Fisher Scientific Cytokeratin 5 (KRT5) Monoclonal Antibody (KRT5, 3594)
인간 KRT5 단백질을 인식하는 Mouse monoclonal 항체로, IHC(P) 및 Peptide Array에 적합합니다. 단백질 A/G로 정제된 액상 형태이며, PBS buffer에 보존제 없이 제공됩니다. 상피세포 마커 연구 및 종양 진단 연구용으로 활용됩니다.
- 판매단위
- pk
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | - |
| Peptide Array (Array) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | KRT5, 3594 |
| Immunogen | Recombinant fragment (around aa 316–590) of human Cytokeratin 5 (KRT5) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage conditions | -20°C or -80°C if preferred |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Cytokeratin pan belongs to a subfamily of intermediate filament proteins characterized by biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40–68 kDa and have an isoelectric pH between 4.9–7.8.
Each human cytokeratin (numbered 1–20) shows expression patterns that reflect epithelial type and differentiation state. These patterns aid in distinguishing different epithelial malignancies.
Cytokeratin antibodies are valuable tools for tumor differential diagnosis using immunohistochemistry, cytopathology, and flow cytometry. Keratin expression varies with cell type, differentiation, growth environment, and disease state, making them useful markers in cancer research and tumor diagnostics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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