Thermo Fisher Scientific CENPO Monoclonal Antibody (OTI7F9), TrueMAB
CENPO 단백질을 인식하는 Mouse monoclonal 항체로 Western Blot에 적합합니다. 인간 유래 단백질 조각(aa 1–230)을 항원으로 제작되었으며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 친화 크로마토그래피로 정제되었고, PBS/BSA/glycerol 완충액에 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7F9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–230 of human CENPO produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Accurate chromosome segregation during mitosis requires a kinetochore to assemble correctly at the centromere of each chromatid and form a dynamic interface with the microtubules of the mitotic spindle.
The kinetochore assembly includes the multisubunit CENP-H/I complex, which can be divided into three functional classes based on phenotype analysis. One of these classes is referred to as the CENP-O class of proteins, of which CENPO is the exemplar. These proteins form a stable complex with each other and are required for proper kinetochore function and for recovery from mitotic spindle damage.
CENPO has also been shown to be an auto-antigen in a small population of anti-centromere antibody (ACA)-positive patients with scleroderma.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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