Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), PerCP-eFluor 710, eBioscience
인간 PD-L1(B7-H1)을 인식하는 MIH1 단클론 항체로, PerCP-eFluor 710 형광 표지. Flow cytometry에 최적화되어 있으며, 488 nm 레이저로 여기, 710 nm에서 방출. 면역억제 관련 PD-1/PD-L1 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (1 µg)/test | View 56 publications |
| ChIP assay (ChIP) | - | View 1 publication |
| Functional Assay (Functional) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MIH1 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11042473 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 786
- eFluor 450
- Functional Grade
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Yellow 700
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- Request custom conjugation
Product Specific Information
Description:
The MIH1 monoclonal antibody reacts with human B7-H1 (PD-L1), a member of the B7 family with a molecular weight of ~40 kDa. B7-H1 is expressed on most leukocytes and serves as a ligand for PD-1, inhibiting T and B cell responses. MIH1 is a reported blocking antibody.
Applications Reported:
Suitable for flow cytometric analysis.
Applications Tested:
Pre-titrated and tested on stimulated human peripheral blood cells. Recommended usage: 5 µL (1 µg) per test in a final volume of 100 µL. Cell number: 10⁵–10⁸ cells/test.
Fluorochrome Information:
PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.
- Excitation: 488 nm (blue laser)
- Emission: 710 nm
- Recommended filters: 685 LP dichroic mirror and 710/40 band pass (695/40 acceptable alternative)
PerCP-eFluor® 710 conjugated antibodies remain stable after exposure to 2% formaldehyde overnight at 4°C.
Filtered post-manufacture through 0.2 µm membrane.
Target Information
PD-L1 (Programmed death receptor ligand 1, B7-H1):
A B7 family member that binds PD-1 receptor, negatively regulating T-cell receptor (TCR) signaling. PD-L1 engagement reduces TCR-mediated proliferation and cytokine production. Highly expressed in various human and murine cancers, upregulated by IFN-γ, and implicated in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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