Thermo Fisher Scientific gp100/Melanosome/PMEL17/SILV Recombinant Rabbit Monoclonal Antibody (PMEL, 1825R)
Recombinant rabbit monoclonal antibody targeting human gp100/PMEL17, ideal for WB and IHC applications. High specificity, no cross-reactivity with dog or rat. Supplied unconjugated, purified via Protein A/G, in PBS with BSA and sodium azide.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PMEL, 1825R |
| Immunogen | Recombinant full-length human gp100 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- This antibody does not react with Dog or Rat.
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Melanoma is a malignant tumor of melanocytes, found mainly in the skin but also in the bowel and eye (including uveal melanoma). It is a rare skin cancer type but responsible for most skin cancer-related deaths.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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