Takara Tet-tTS transcriptional silencer

pTet-tTS expresses the tetracycline/doxycycline-controlled transcription silencer (tTS), which is a fusion of the tet repressor protein (TetR) with a KRAB silencing domain. tTS can be used to significantly lower the basal expression from first-generation Tet systems, but is not required for Tet-On 3G or Tet-One systems since the basal expression is typically very low.

pTet-tTS expresses the tetracycline/doxycycline-controlled transcription silencer (tTS), which is a fusion of the tet repressor protein (TetR) with a KRAB silencing domain. tTS can be used to significantly lower the basal expression from first-generation Tet systems, but is not required for Tet-On 3G or Tet-One systems since the basal expression is typically very low.

In the absence of doxycycline, tTS binds to the tet-operator sequences within TRE promoters, silencing gene expression. Upon addition of doxycycline, tTS undergoes a conformational change that causes its release from the promoter, to be replaced by the Tet-On transactivator.

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Overview

• Expresses a fusion of the tet repressor protein (TetR) with a KRAB silencing domain
• Reduced basal expression when combined with first-generation Tet-On systems
• Active suppression of transcription when bound to TRE promoters
• Binds in the absence of doxycycline
• Not required for third-generation Tet systems

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