Thermo Fisher Scientific PKC epsilon Recombinant Rabbit Monoclonal Antibody (4T6M8)
PKC epsilon 단백질을 인식하는 Rabbit Recombinant Monoclonal Antibody (Clone 4T6M8). Western blot 및 ELISA에 적합하며, 높은 특이성과 재현성을 제공. Human, Mouse, Rat 시료에 반응하며, 단백질 A로 정제된 액상 형태.
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Thermo Fisher Scientific PKC epsilon Recombinant Rabbit Monoclonal Antibody (4T6M8)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4T6M8 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 488–737 of human PKCε |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.43 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
Product Specific Information
Immunogen Sequence:
YVNGGDLMFQ IQRSRKFDEP RSRFYAAEVT SALMFLHQHG VIYRDLKLDN ILLDAEGHCK LADFGMCKEG ILNGVTTTTF CGTPDYIAPE ILQELEYGPS VDWWALGVLM YEMMAGQPPF EADNEDDLFE SILHDDVLYP VWLSKEAVSI LKAFMTKNPH KRLGCVASQN GEDAIKQHPF FKEIDWVLLE QKKIKPPFKP RIKTKRDVNN FDQDFTREEP VLTLVDEAIV KQINQEEFKG FSYFGEDLMP
Target Information
The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action, response to extracellular signals, and substrate specificity.
PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and plays a distinct role in cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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