
Thermo Fisher Scientific PRKAR2A Monoclonal Antibody (OTI1A7), TrueMAB
Human PRKAR2A 단백질을 인식하는 mouse monoclonal antibody로 Western blot, IHC, ICC/IF, Flow cytometry 등에 사용 가능. Lyophilized 형태이며 PBS buffer에 trehalose 함유. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A7 |
| Immunogen | Full length human recombinant protein of human PRKAR2A produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
cAMP is a signaling molecule important for various cellular functions. It activates cAMP-dependent protein kinase (AMPK), which phosphorylates target proteins. The inactive holoenzyme of AMPK is a tetramer of two regulatory and two catalytic subunits. Upon cAMP binding, the holoenzyme dissociates into regulatory dimers and catalytic monomers.
The PRKAR2A gene encodes one of the regulatory subunits, which can be phosphorylated by the activated catalytic subunit. It may interact with A-kinase anchoring proteins and determine AMPK subcellular localization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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