Thermo Fisher Scientific KIT Monoclonal Antibody (6F2)

Applications

Western Blot (WB)

• Tested Dilution: 1–5 µg/mL

ELISA

• Tested Dilution: 0.03 ng/mL

Product Specifications

Product Specific Information

Sequence of this protein is as follows:
PGKSDLIVRV GDEIRLLCTD PGFVKWTFEI LDETNENKQN EWITEKAEAT NTGKYTCTNK HGLSNSIYVF VRDPAKLFLV DRSLYGKEDN DTLVRCPLTD

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT and its ligand regulate growth and activation of various hemopoietic and non-hemopoietic cells.

Mutations in KIT are associated with:

• Gastrointestinal stromal tumors
• Mast cell disease
• Acute myelogenous leukemia
• Piebaldism

Deregulation of KIT receptor TK by the prevalent activation loop mutation D816V has become a focal point in therapeutic strategies for neoplastic mast cell growth.

Expression:
c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract cajal cells. Upon binding of stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of tyrosine residues including Tyr703. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream pathways such as PI3K, PLC-γ, and JAK/STAT.

KIT receptor activation is essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.