Thermo Fisher Scientific 6x-His Tag Polyclonal Antibody, HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:200-1:500

-

ELISA (ELISA)

1:10,000-1:100,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

6-His (HHHHHH) conjugated to KLH

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.05% Pro-Clean 400

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_557408

Product Specific Information

This antibody does not detect all N-terminal 6-His tagged proteins.

Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at
280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme/antibody protein ratio is 4:1.

Target Information

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.