Thermo Fisher Scientific SYBR Green Fast Advanced Cells-to-CT Kit, 40 reactions

Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit

The SYBR Green Fast Advanced Cells-to-CT Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification. It provides high sensitivity and the fastest workflow available—obtain gene expression results from an entire plate of cells in about 1.5 hours.

주요 특징

• Complete — includes optimized cell lysis reagents with gDNA removal, Fast Advanced RT enzyme mix, buffer, and PowerUp SYBR Green Fast Advanced Master Mix
• Fast — 7-minute sample prep, including DNase treatment, at room temperature
• Easy — lyse samples in a tube or directly in culture plates
• Robust — supports 10–100,000 cells per sample; results equivalent to purified RNA
• Efficient — sufficient for up to 1000 real-time PCR results from 100 samples

Unique method eliminates the need to isolate RNA

The Cells-to-CT method uses a unique lysis reagent that lyses cells and protects RNA. DNA removal occurs simultaneously in the 5-minute lysis step. Lysis is stopped by adding a Stop Solution, which irreversibly binds to the lysis reagents.

The kit includes:

• Cells-to-CT Fast Advanced reverse transcription (RT) reagents for cDNA synthesis
• PowerUp SYBR Green Master Mix for real-time PCR analysis
• (Primer sets are provided by the user.)*

Simple 7-minute sample preparation

Designed for 10–100,000 cultured cells per sample.

1. Wash cells in PBS.
2. Lyse in lysis solution for 5 minutes at room temperature (optional DNase treatment).
3. Stop reaction with Stop Solution for 2 minutes at room temperature.

Lysates are ready for reverse transcription or can be stored at –20°C (short-term) or –80°C (long-term).

Maximize sensitivity of detection

The kit allows up to 45% of the RT reaction to be cell lysate and 30% of the PCR reaction to be cDNA, maximizing sensitivity. The PowerUp SYBR Green Master Mix ensures precise amplification of even low-abundance transcripts.

Reduced sample loss and transfer error

Samples can be processed directly in culture wells (96 or 384 wells), minimizing handling and transfer errors. No heating, washing, or centrifugation steps are required, reducing a 30–60 minute process to just 7 minutes.

Proven performance, proven together

All components are optimized for consistent and reliable performance, eliminating the need to assemble separate kits. Achieve high sensitivity with the fastest sample-to-answer workflow available.

사양 (Specifications)

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