Thermo Fisher Scientific PRKAR2A/PKA-RIIalpha Polyclonal Antibody
PRKAR2A/PKA-RIIalpha 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, WB, IHC, IP 등에 사용 가능. 인간 시료 반응성, 항원 친화 크로마토그래피 정제, 액상 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 50 and 100 of Human protein kinase, cAMP-dependent, regulatory, type II, alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
cAMP is a signaling molecule important for various cellular functions. It activates the cAMP-dependent protein kinase (AMPK), which phosphorylates target proteins. The inactive holoenzyme of AMPK consists of two regulatory and two catalytic subunits. Upon cAMP binding, the holoenzyme dissociates into regulatory dimers bound to cAMP and free catalytic monomers. Humans possess four regulatory and three catalytic subunits of AMPK. The protein encoded by this gene is one of the regulatory subunits, which can be phosphorylated by the catalytic subunit and interacts with A-kinase anchoring proteins to determine AMPK localization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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