Thermo Fisher Scientific E4F1 Monoclonal Antibody (4B10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

ELISA (ELISA)

0.1 ng/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

4B10

Immunogen

E4F1 (NP_004415.2, 685 a.a. approximately 784 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:E4F1,uniProtId:Q66K89-1,ncbiNodeId:9606,antigenRange:685-784,antigenLength:784,antigenImageFileName:H00001877-M03_E4F1_Q66K89-1_House_mouse.svg,antigenImageFileNamePDP:H00001877-M03_E4F1_Q66K89-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: GHQIIVQNVT MDEETALGPE AAAADTITIA TPESLTEQVA MTLASAISEG TVLAARAGTS GTEQATVTMV SSEDIEILEH AGELVIASPE GQLEVQTVIV

Target Information

May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription.; FUNCTION: Identified as a cellular target of the adenoviral oncoprotein E1A, it is required for both transcriptional activation and repression of viral genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.