Thermo Fisher Scientific Phospho-PDHA1 (Ser293) Recombinant Rabbit Monoclonal Antibody (1Q9I5)
Phospho-PDHA1 (Ser293) 항체는 인산화된 PDHA1 단백질을 검출하기 위한 재조합 토끼 단클론 항체입니다. Western blot과 ELISA에 적합하며, 높은 특이성과 재현성을 제공합니다. Human과 Mouse 시료에 반응하며, 연구용으로 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:20,000
ELISA
- Tested Dilution: 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 1Q9I5 |
| Immunogen | A synthetic phosphorylated peptide around S293 of human Phospho-PDHA1-S293 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.65 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
Product Specific Information
- Immunogen Sequence: GHSMS
Target Information
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the conversion of pyruvate to acetyl-CoA and CO₂, linking glycolysis to the tricarboxylic acid (TCA) cycle.
The PDH complex consists of multiple copies of three enzymatic components:
- Pyruvate dehydrogenase (E1)
- Dihydrolipoamide acetyltransferase (E2)
- Lipoamide dehydrogenase (E3)
The E1 enzyme is a heterotetramer composed of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, essential for PDH complex function. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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