Thermo Fisher Scientific ISG15 Polyclonal Antibody
Human ISG15 단백질을 인식하는 Rabbit Polyclonal Antibody로 다양한 면역실험(WB, IHC)에 적합. KLH 결합 합성 펩타이드(31–61 aa)를 면역원으로 사용. 고순도 Protein A 및 항원 친화 크로마토그래피 정제. 안정적 액상 형태로 제공되며 장기 보관 시 -20°C 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 31–61 amino acids from the N-terminal region of human ISG15 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2126208 |
Target Information
Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein.
It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and augmentation of non-MHC-restricted cytotoxicity.
Synthesis is stimulated by IFN-alpha, IFN-beta, or IFN-omega, but not IFN-gamma.
ISG15 expression is induced by overexpression of interferon regulatory factors and by influenza B virus.
ISG15 is secreted by monocyte, T-lymphocyte, B-lymphocyte, human fibroblast, and epithelial cell lines.
Enhancement of NK cell proliferation and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade, suggesting its role in amplifying immunomodulatory effects of interferons.
Mutations affecting the gene can result in Immunodeficiency 38 with basal ganglia calcification (IMD38).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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