Thermo Fisher Scientific Cas9 Recombinant Rabbit Monoclonal Antibody (5C2Q1)
Cas9 단백질을 특이적으로 인식하는 Thermo Fisher의 재조합 토끼 단클론 항체. Western blot 및 ELISA에 적합하며, 높은 특이성과 재현성을 제공. HEK293 세포 발현 시스템에서 생산된 IgG 형식의 액상 항체로, 안정적 보관이 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria, Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 5C2Q1 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 450–730 of Streptococcus pyogenes m1 Cas9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.75 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
Product Specific Information
Immunogen Sequence:
YYVGPLARGN SRFAWMTRKS EETITPWNFE EVVDKGASAQ SFIERMTNFD KNLPNEKVLP KHSLLYEYFT VYNELTKVKY VTEGMRKPAF LSGEQKKAIV DLLFKTNRKV TVKQLKEDYF KKIECFDSVE ISGVEDRFNA SLGTYHDLLK IIKDKDFLDN EENEDILEDI VLTLTLFEDR EMIEERLKTY AHLFDDKVMK QLKRRRYTGW GRLSRKLING IRDKQSGKTI LDFLKSDGFA NRNFMQLIHD DSLTFKEDIQ KAQVSGQGDS LHEHIANLAG S
Target Information
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering.
This system uses RNA to guide the nuclease to a specific DNA sequence, allowing for targeted genome editing such as indel mutations, sequence insertions, deletions, or rearrangements.
Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9).
SaCas9 is smaller (1053 AA vs. 1368 AA for SpCas9), highly efficient, and recognizes an NNGRRT PAM sequence, making it suitable for compact delivery systems such as AAV vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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