Thermo Fisher Scientific Tmem119 Monoclonal Antibody (V3RT1GOsz), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test
• View 8 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Brilliant UV 805
• Brilliant Violet 421
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This V3RT1GOsz monoclonal antibody recognizes mouse Tmem119 and has been validated for flow cytometry.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse brain cells after myelin removal; recommended ≤1.0 µg/test (100 µL final volume).
• Cell Density: 10⁵–10⁸ cells/test (empirically determined).
• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Compatible with IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57). Samples can be stored up to 3 days at 4°C in the dark.
• Excitation/Emission: 488–561 nm / 775 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Target Information

Tmem119 encodes a single-pass type I transmembrane protein interacting with micro-RNAs and transcription factors (SMAD1, SMAD5, RUNX2).
It serves as a microglia-specific surface marker distinguishing microglia from infiltrating macrophages.
Microglia are brain-resident phagocytes involved in debris clearance and synaptic pruning, showing functional plasticity in neuroprotective or neurotoxic responses.
Reactive microglia co-express Iba1 and can induce inflammatory A1 astrocytes via secretion of Cq1, IL-1a, and TNF, contributing to neurodegenerative diseases such as Alzheimer’s, Parkinson’s, Huntington’s, and Multiple Sclerosis.
Outside the nervous system, Tmem119 is implicated in osteoblast differentiation and ossification.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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