Thermo Fisher Scientific Human RARA, Ligand Binding Domain, GST Tag Recombinant Protein

Applications

• Ligand Binding Assay (LBA): Assay-dependent

Product Specifications

Product Specific Information

Retinoic acid receptor alpha, ligand binding domain (RARA-LBD) is a 59.53 kDa recombinant human protein (amino acids 176–462) expressed as a GST-tagged fusion protein in baculovirus-infected insect cells.
RARA-LBD is expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator recruitment studies.

Protein Form: Recombinant, Ligand Binding Domain

Target Information

RARA is a receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands (all-trans or 9-cis retinoic acid) and regulate gene expression in various biological processes.
The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1–DR5.
In the absence of ligand, the RXR-RAR heterodimers associate with transcription corepressors that induce histone acetylation, chromatin condensation, and transcriptional suppression.
Upon ligand binding, the corepressors dissociate and coactivators are recruited, leading to transcriptional activation.

RARA plays an essential role in retinoic acid-induced germ cell development during spermatogenesis, supports survival of early spermatocytes, and contributes to skeletal growth and matrix homeostasis. It regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing KMT2E/MLL5, mediating retinoic acid-induced granulopoiesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.