Thermo Fisher Scientific DCAF4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human DCAF4. Recombinant protein control fragment (Product #RP-100870). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DCAF4,uniProtId:Q8WV16-1,ncbiNodeId:9606,antigenRange:53-116,antigenLength:495,antigenImageFileName:PA5-61485_DCAF4_Q8WV16-1_Rabbit.svg,antigenImageFileNamePDP:PA5-61485_DCAF4_Q8WV16-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2640408

Product Specific Information

Immunogen sequence: TSSGTAGTSS VPELPGFYFD PEKKRYFRLL PGHNNCNPLT KESIRQKEME SKRLRLLQEE DRRK

Highest antigen sequence identity to the following orthologs: Mouse - 81%, Rat - 80%.

Target Information

The nuclear protein p100 is a proliferation-associated protein whose expression is restricted to cell cycle phases S, G2, and M. Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. The full-length cDNA encodes a 747-amino acid protein with a putative ATP/GTP-binding site motif. RT-PCR analysis demonstrated strong expression of in lung carcinoma cell lines but not in normal lung tissues. Expression was also found in adult placenta, skeletal muscle, thymus, testis, and small intestine and in fetal brain, liver, and kidney. P100 is also correlated to cancer prognosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.