Thermo Fisher Scientific MCU Polyclonal Antibody

Applications

• Western Blot (WB): Tested dilution 1:200

Product Specifications

Product Specific Information

• Reconstitute antibody with 50 µL of double distilled water (DDW).
• The reconstituted solution can be stored at 4°C for up to 1 week.
• For longer storage, aliquot and keep at -20°C.
• Avoid multiple freeze–thaw cycles.
• Centrifuge all antibody preparations before use (10,000 × g, 5 min).
• Peptide sequence: CIEQHQLNKE RELIER
• Homology: Rat, mouse – 15/16 amino acid residues identical.

Target Information

Mitochondrial inner membrane calcium uniporter (MCU) mediates calcium uptake into mitochondria. It forms the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex).
Activity is regulated by MICU1 and MICU2:

• At low Ca²⁺ levels, MCU activity is down-regulated by MICU1 and MICU2.
• At higher Ca²⁺ levels, MICU1 increases MCU activity.

Mitochondrial calcium homeostasis plays key roles in:

• Cellular physiology and bioenergetics
• Cytoplasmic calcium signaling
• Activation of cell death pathways

Additional roles include:

• Buffering systolic calcium rises in cardiomyocytes
• Regulating mitochondrial calcium loading during acute stress ("fight-or-flight" response)
• Participating in mitochondrial permeability transition during ischemia–reperfusion injury
• Regulating glucose-dependent insulin secretion in pancreatic β-cells
• Influencing muscle size in skeletal muscle cells
• Regulating synaptic vesicle endocytosis kinetics in central nerve terminals
• Involvement in antigen processing and presentation

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.