Thermo Fisher Scientific PKMYT1 Monoclonal Antibody (OTI2B4), TrueMAB
PKMYT1 단백질을 인식하는 마우스 단클론 항체로 Western blot, ICC/IF, 유세포분석에 사용 가능. HEK293T 세포에서 발현된 재조합 단백질을 면역원으로 제작. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용. 연구용 전용 제품.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:200 | |
| Immunocytochemistry (ICC/IF) | Assay-dependent | |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B4 |
| Immunogen | Full length human recombinant protein of human PKMYT1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
PKMYT1 serine/threonine kinase is a neural-specific, zinc finger-containing DNA-binding protein that binds to promoter regions of proteolipid proteins in the central nervous system, playing a key role in neural development.
MYT1, along with related CCHC zinc finger proteins, is expressed in developing neurons. Recombinant PKMYT1 fragments containing zinc finger domains bind the same cis regulatory element in the PLP1 promoter in vitro, with DNA binding requiring Zn²⁺ but not other divalent cations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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