Thermo Fisher Scientific SPRED1 Monoclonal Antibody (462CT2.5.1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:250

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

462CT2.5.1

Immunogen

purified His-tagged SPRED1 protein (fragment)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897494

Target Information

The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes; it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.